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Then, the PBS was added to the sample deposit, mixed it, and centrifuged

Lower concentrations produce slower or less noticeable results

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Cancer Res 63 : 627635 Sciacca L, Mineo R, Pandini G, Murabito A, Vigneri R, Belfiore A (2002) In IGF-I receptor-deficient leiomyosarcoma cells autocrine IGF-II induces cell invasion and protection from apoptosis via the insulin receptor isoform A